growth inhibitory impact of peganum harmala l. on two breast cancer cell lines
نویسندگان
چکیده
background: ß-carbolines, harmaline and harmine, are the major alkaloids presenting in the seeds of the peganum harmala l. these alkaloids are known as herbal active principals with potential use in pharmaceutical and medicine. objectives: to assess the growth inhibitory effect of phyto-alkaloids, harmaline and harmine, on cancer cell lines. methods: the p. harmala l.’s alkaloids were extracted by acidic/basic extraction method and identified by two methods, fourier transform infra-red spectroscopy (ftir) and high performance liquid chromatography (hplc). two breast cancer cell lines, mda_mb_231, mcf-7, were subjected to different concentration (1–100 μg.ml-1) of the p. harmala extract at different time courses (24h, 48h, and 72 h). methylthiazol tetrazolium (mtt) test, the half maximal inhibitory concentration (ic50) and the morphological changes through optical microscopy were evaluated. results: in both studied cell lines, the p. harmala extract decreased cell viability in longer time exposure in a dose dependent manner. the more concentrated extract led to higher motility of mda-mb-231 at 24h. although, mcf-7 cell line required longer exposure time has to reach the same motility. it was observed that 30 μg.ml-1 is the minimum lethal dose that kills approximately 50% of cells at 24 hours in mda-mb-231 cell line (ic50). ic50 for mcf-7 were calculated 40 μg.ml-1 and 25 μg.ml-1 at 48 h and 72 h, respectively. the morphological observation ensured the apoptosis nature of p. harmala on cells as their membrane kept intact and no membrane permeabilization was observed. conclusions: the results revealed that the p. harmala extracts decreased significantly growth rate and cell survival of cancer cell lines. the extract induced cell death regarding natural cell growth rate. mda-mb-231 cell line naturally has a higher growth rate than mcf-7 cell line, so higher growth inhibition of mda-mb-231 cell line by the p. harmala extract was confirmed.
منابع مشابه
Growth Inhibitory Impact of Peganum harmala L. on Two Breast Cancer Cell Lines
Background: ß-carbolines, harmaline and harmine, are the major alkaloids presenting in the seeds of the Peganum harmala L. These alkaloids are known as herbal active principals with potential use in pharmaceutical and medicine. Objectives: To assess the growth inhibitory effect of phyto-alkaloids, harmaline and harmine, on cancer cell lines. Method...
متن کاملPeganum harmala L.’s anti-growth effect on a breast cancer cell line
This research was done to evaluate the induction of apoptosis in MDA-MB-231 breast cancer cell line by Peganum harmala's extract, in which a significant amount of ß-carbolines is included. The apoptosis incidence was assessed through Annexin-V-Flous kit. The expressions of genes through which intrinsic apoptosis pathway are involved, Bax, Bcl-2, Bid, and Puma, over the genes the expressions of ...
متن کاملPeganum harmala L. Intoxication in a Pregnant Woman
Peganum harmala L. is a plant widely distributed in the Mediterranean region. It is commonly used in traditional medicine in Morocco as sedative and abortifacient but exposes users to the risk of overdose and poisoning. The pharmacologically active compounds of this plant include a number of β -carboline and quinazoline alkaloids responsible of its pharmacological and toxicological effects. We ...
متن کاملCytotoxic Indole Alkaloids against Human Leukemia Cell Lines from the Toxic Plant Peganum harmala
Bioactivity-guided fractionation was used to determine the cytotoxic alkaloids from the toxic plant Peganum harmala. Two novel indole alkaloids, together with ten known ones, were isolated and identified. The novel alkaloids were elucidated to be 2-(indol-3-yl)ethyl-α-L-rhamnopyranosyl-(1 → 6)-β-D-glucopyranoside (2) and 3-hydroxy-3-(N-acetyl-2-aminoethyl)-6-methoxyindol-2-one (3). The cytotoxi...
متن کاملThe Inhibitory Effects of Ascorbic Acid, ?-Tocopherol, and Sodium Selenite on Proliferation of Breast Cancer Cell Lines
The role of antioxidants in prevention and treatment of cancers have been reported by several studies. In our investigation we studied the effects of ascorbic acid, ?-tocopherol, and sodium selenite on proliferation of two breast cancer cell lines: T47D (estrogen-receptor positive) and MDA-MB-231 (estrogen-receptor negative). We also used 17-?-estradiol as positive control for proliferation of ...
متن کاملBiotransformation of Aromatic Aldehydes by Cell Cultures of Peganum harmala L. and Silybum marianum (L.) Gaertn.
Many aldehydes are important components of natural flavours. They are used in food, cosmetic, and biomedical industries in large amounts. Plant cells or microorganisms carry out their production by biotransformation, which is one of the biotechnological methods that allow them to be defined as 'natural'. Cell cultures of Silybum marianum and Peganum harmala have been studied with a view to in...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
iranian journal of biotechnologyناشر: national institute of genetic engineering and biotechnology
ISSN 1728-3043
دوره 12
شماره 1 2014
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023